NIHR Sheffield Clinical Research Facility. Venepuncture using Vacutainer and Syringe Butterfly
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1 * STANDARD OPERATING PROCEDURE NIHR Sheffield Clinical Research Facility Venepuncture using Vacutainer and Syringe Butterfly SOP Histry (archived date) V2.0 (21/05/2013) reviewed by Michael Agyemang; V1.3 (01/11/2010); V1.2 (25/02/2010); V1.1 (20/01/2009); V1.0 (24/11/2006) SOP Number CRF.C112 Created by Alisn Mrtimer Versin V3.0 Date Review Date Octber 2018 Related SOPs 1 R&D.A106, R&D.A117 Apprved by Jhn Humphreys SOP CRF.112 Versin 3.0 Page 1 f 6 *The master dcument is available n the CRF I:\ drive and website: print-ff f this dcument by anyne ther than the CRF SOP C-rdinatr will be classed as uncntrlled. Researchers and their teams may print ff this dcument fr training and reference purpses but are respnsible fr regularly checking with the CRF Sp C-rdinatr fr the mst recent versin.
2 Standard Operating Prcedure: NIHR Sheffield Clinical Research Facility Venepuncture using Vacutainer & Syringe Butterfly This SOP has been written t give general guidance t study persnnel n perfrming venepuncture. There are n GCP Guidelines cncerning venepuncture. Backgrund It is imprtant that all staff in the CRF perfrm venepuncture t Sheffield Teaching Hspitals NHS Fundatin Trust standards t ensure participant and staff safety. Definitin Venepuncture is the term used fr the prcedure f entering a vein with a needle. Venepuncture is carried ut fr 2 reasns: 1. T btain a bld sample f diagnstic purpse. 2. Mnitr levels f bld cmpnents. The CRF at RHH refers t the Clinical Research Facility, O Flr, Ryal Hallamshire Hspital. The CRF at NGH refers t the Clinical Research Facility, 1 st Flr, Centre fr Bimedical Research, Nrthern General Hspital. Prcedure A (Vacutainer Butterfly) 1. The investigatr is respnsible fr perfrming venepuncture accrding t prtcl. This duty can be delegated t ther apprpriately qualified members f the research team as recrded n the Prject Delegatin Lg. 2. Prir t the prcedure the investigatr r delegated persn must ensure the crrect participant is identified and crss referenced with apprpriate request frms. The areas t be checked are surname, frename, date f birth, research study name and study subject number if apprpriate, hspital registratin number and current address. 3. The investigatr r delegated persn must perfrm the prcedure in accrdance with the trust relevant Health and Safety Plicy. 4. The investigatr r delegated persn must ensure cnstant attentin t an apprpriate aseptic technique whilst prcedure is being perfrmed as identified in the Sheffield Infectin Cntrl Guidelines. 5. The investigatr r delegated persn will refer t the prtcl t ensure specific requirements fr perfrming venepuncture are identified. 6. The investigatr r delegated persn must ensure that the patient has been prepared fr any specific bld tests, e.g. fasting blds. 7. The investigatr r delegated persn will explain the prcedure t the participant. 8. The investigatr r delegated persn shuld ensure the participant has had n previus prblems when having bld taken and if they have any knwn allergies t tape. 9. The investigatr r delegated persn shuld wash their hands accrding t STHFT Hand Hygiene Plicy. SOP CRF.112 Versin 3.0 Page 2 f 6
3 10. The investigatr r delegated persn will clean and prepare trlley/wrk area. 11. The investigatr r delegated persn will prepare all equipment required ensuring all study specific equipment is btained. 12. Equipment required: Sharps bin Vacutainer butterfly needle r syringe butterfly needle Vacutainer bdy Syringe Bld transfer unit if using syringe Vacutainer bttles/study specific bld kits Mediswab Cttn wl swab Micrpre tape Plaster Nn-sterile glves Turniquet Trlley Plaster 13. The investigatr r delegated persn will ensure all study specific dcumentatin is prepared and any lcal request frms are cmpleted. 14. The investigatr r delegated persn must wash their hands accrding t STHFT Hand Hygiene Plicy and check fr brken skin and cver with waterprf dressing. 15. The investigatr r delegated persn will apply glves. 16. The investigatr r delegated persn will lsely apply turniquet. Psitin f the turniquet may be varied depending n where the sample is t be btained frm. 17. The investigatr r delegated persn will ask the participant t rest their arm n an arm rest (if available) r place a pillw under their elbw. 18. The investigatr r delegated persn will tighten the turniquet and assess the patients arm fr suitable veins. If necessary, access the participants ther arm until a suitable vein is identified. SOP CRF.112 Versin 3.0 Page 3 f 6
4 19. The investigatr r delegated persn shuld nt make mre than 3 attempts t btain a venus sample. 20. The investigatr r delegated persn must prepare participants skin with a mediswab fr at least 30 secnds (allw the skin t dry). D nt repalpate the vein r tuch the skin. 21. The investigatr r delegated persn will remve the vacutainer white sheath and dispse. 22. The investigatr r delegated persn shuld remve the white end f the vacutainer luer lck cnnecter and cnnect t the butterfly hub cnnectr. 23. The investigatr r delegated persn will remve the sheath frm the butterfly needle and place in a receptacle. 24. The investigatr r delegated persn must anchr the skin by applying manual tractin a few centimetres belw the prpsed insertin site. 25. The investigatr r delegated persn must fllw the line f the vein and insert the needle thrugh the skin at an angle f 15 degrees, ensuring the eye f the needle is cmpletely in the vein t prevent haematma frmatin and/r bld leakage. 26. The investigatr r delegated persn must ensure the vacutainer bdy and needle is kept still, and attach the sample tubes as apprpriate. If necessary, release turniquet befre all samples are taken. 27. Once all the samples are taken the investigatr r delegated persn will release the turniquet if nt already dne. 28. The investigatr r delegated persn will place a cttn wl ball swab ver the puncture site. 29. The investigatr r delegated persn must remve the needle and apply sufficient pressure t prevent bleeding (D nt apply pressure until the needle has been cmpletely remved). If able, the investigatr r delegated persn can ask the participant t maintain pressure fr 2 minutes. 30. Once all the samples are taken the investigatr r delegated persn must dispse the needle and syringe as a whle unit int an apprpriate sharps bin in accrdance with STHFT Waste Strategy & Plicy. The needle must nt be re sheathed prir t dispsal. 31. If any bld spillage ccurs the investigatr r delegated persn shuld clean up the spillage in accrdance with STHFT plicy using the apprpriate spillage kits. The spillage kits are lcated in the Phlebtmy Rm and Labratry in the CRF at RHH and in the Labratry and Dirty Utility Rm in the CRF at NGH. 32. The investigatr r delegated persn must mix all the specimens well if the specimens cntain an additive r as dictated by the prtcl. 33. The investigatr r delegated persn must inspect the puncture site befre applying the dressing. The cttn swab shuld be taped dwn firmly. SOP CRF.112 Versin 3.0 Page 4 f 6
5 34. The investigatr r delegated persn must ensure the participant is cmfrtable. 35. The investigatr r delegated persn must ensure all the samples are crrectly labelled. 36. The investigatr r delegated persn will ensure all prcedures fr the btained samples utlined in the study prtcl are fllwed. 37. If the samples are fr STH labs the investigatr r delegated persn must ensure prmpt transprt t the apprpriate labratries. 38. The investigatr r delegated persn must remve glves and discard int apprpriate clinical waste bag. 39. The investigatr r delegated persn must wash their hands in accrdance with STHFT Hand Hygiene Plicy. 40. The investigatr r delegated persn must recrd all samples btained n the relevant surce dcumentatin fr the study r in the participant s medical ntes. Prcedure B (Syringe butterfly) 1. Repeat 1 20 abve 2. The investigatr r delegated persn remves the end f the butterfly needle cnnectr and attach the butterfly needle t the apprpriate size syringe. 3. The investigatr r delegated persn will remve the sheath frm the butterfly needle and place in a receptacle. 4. The investigatr r delegated persn must anchr the skin by applying manual tractin a few centimetres belw the prpsed insertin site. 5. The investigatr r delegated persn must fllw the line f the vein and insert needle thrugh the skin at an angle f 15 degrees, ensuring the eye f the needle is cmpletely in the vein t prevent haematma frmatin and/r bld leakage. 6. The investigatr r delegated persn must ensure the butterfly needle and syringe is kept steady and slwly withdraws the required vlume f bld. 7. Once all the samples are taken the investigatr r delegated persn will release the turniquet if nt already dne. 8. The investigatr r delegated persn must detach the butterfly needle frm the syringe and dispse f it int apprpriate yellw sharps bin. 9. The investigatr r delegated persn must attach a bld transfer unit t the syringe and insert int bld specimen tubes and allw the apprpriate amunt f bld t be drawn in. 10. The investigatr r delegated persn must dispse f the transfer unit and the syringe as a whle int an apprpriate yellw sharps bin in accrdance with STHFT waste Strategy and Plicy. 11. Repeat abve SOP CRF.112 Versin 3.0 Page 5 f 6
6 Related Dcumentatin 1 Dcument Name Prject Delegatin Lg STH Hand Hygiene Plicy STHFT Health and Safety at Wrk Plicy Statement STHFT Infectin cntrl guidelines Bld and Bdy Fluid Expsure Incident Management Pack STHFT venepuncture and intravenus cannulatin pen learning prgramme STHFT Plicy fr the Decntaminatin f Hspital equipment & medical devices Sheffield Waste Strategy & Plicy Authr CRF STH Infectin Cntrl Team Mr N Riley Mrs A Redfern STH Infectin Cntrl Team STH Fundatin Trust STH Fundatin Trust STH Fundatin Trust J Watts & M Mahn 1 The lcatin(s) f any related dcument(s) are listed in the CRF SOP Referenced Dcuments Directry. The CRF SOP Referenced Dcument Directry and any Related SOPs, listed n page 1 f this SOP, can be accessed electrnically at r can be requested by cntacting Sheffield Clinical Research Facility, O Flr, Ryal Hallamshire Hspital, Glssp Rad, Sheffield, S10 2JF, SOP CRF.112 Versin 3.0 Page 6 f 6
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