FSHN Safety Seminar. Jeanne Stewart. August 26, 2015

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1 FSHN Safety Seminar Jeanne Stewart August 26, 2015

2 FSHN Safety News Safety checklist for a new graduate student or student employee to begin Safety checklist for new FACULTY to begin work work in a in lab: a lab: Obtain ISU identification card (ISUCard) from 0530 Beardshear Hall. Register for NetID to obtain account ( Obtain or go ISU to the identification Solution Center card at 195 (ISUCard) Durham Center. from 0530 Beardshear Hall. Activate your status Register and provide for NetID contact to information obtain for emergency account ( Plus. or go to the Solution Center at 195 Durham Center. notification through ISU Alert using Access Read the Laboratory Activate Safety your Manual status (LSM) and provide contact information for emergency notification ( and place a copy in the lab through ISU Alert using Access Plus. if you lab computer is not connected to the internet. Keep a lab safety binder for safety records in the lab Obtain or a central, keys for accessible lab and location building with from proper Department signs. administrator. Write Standard Operating Procedures (SOP) for all lab methods using EH&S template Work with major professor or lab supervisor to fill out a Hazard Inventory form and place into lab safety binder ( ( Obtain Personal ory.pdf) Protective and equipment send to (PPE) EH&S. as required for SOPs (at least nitrile gloves, labcoats, safety goggles/glasses, First Aid kit, spill kit and secondary containment for unwanted materials) Review notebook/record keeping practices and lab meeting presentation schedule with As you acquire chemicals, major professor. compile a chemical inventory using the EHS template ( and obtain Safety Data Sheets Read Laboratory Safety Manual (SDSs) for each chemical ( ( Write a Lab Site-Specific Safety Training form using the EHS template ( Perform a safety training Needs Assessment through EH&S Write and complete ( Lab Check-in ( and using Complete online &/or classroom safety training as required from Needs Assessment the EHS templates results. Perform lab safety surveys as appropriate using EH&S form ( Undergo Lab site-specific Training through major professor or lab supervisor. Develop an emergency ( action plan using the template from EH&S ( Record safety training in Training History form Fill out a Hazard ( Inventory ( y.pdf) and send to Complete EH&S. a Lab Check-in form through major professor or lab supervisor. Establish notebook/record ( keeping practices for your students. Perform online &/or After classroom project(s) safety assignment, training required undergo from training Needs Assessment for each laboratory method and review through EHS ( SOP with major professor or lab supervisor. Must have record of training for each Record your safety procedure training in signed the Training and dated. History form ( Keep copies of all safety training that you give to all of your graduate students and undergraduate employees. Safety Checklist for New Faculty to begin work in a lab New Staff, Graduate Student or student employee to begin work in a lab Exception undergrad may not have keys

3 FSHN Safety News Safety Training Curriculum for Laboratory Personnel Lab Safety Orientation Complete Core required courses Specific lab Additional Training EHS Research Support Checklist -Setting up a lab - /forms/labcheck.pdf

4 FSHN Safety News Laboratory Safety Training History Keep track while you are here at ISU

5 FSHN Safety News Laboratory Safety Manual SOP, ordering and receiving chemicals & hazardous materials Emergency planning Equipment Training General/specific safety practices Waste and recycling Exposure Assessment & medical care

6 FSHN Safety News Emergency Action Plan Fire Medical Emergencies Urgent Situations Violent Incident Sever Weather Utility Outages Chemical spills (small & large) Radiological Spills (Major) Radiological Contamination Missing Radiological Inventory Biological Spill BBP Exposure

7 FSHN Safety News General Safety Inspection Documentation Fire & Life Safety General Safety Equipment Chemicals & Storage laboratory/forms/surveyforms

8 FSHN Safety News Emergency Evacuation Map

9 FSHN Safety News Importance of current training relative to accidents Training status is ALWAYS the first investigated item when looking into the cause and prevention of accidents. Training is easy to verify and an easy place to point blame. Regulators are not forgiving when it comes to training. Occupational trainings and the frequency of training is mandated in regulatory rule EHS makes it easy to meet the requirement(s).

10 FSHN Safety News Accidents that have happened while performing research in a lab Blood splash up on lip of nurse taking blood-had phlebotomist and participant tested for HIV Student using a new water quality kit and splashed a few drops of chemical on their hand-started to blister. Acid spills that eat through clothing to skin below Salmonella inoculum splash into researcher s eye (could have died with the level s of Salomella that they were working with) Pregnant technician incorrectly secured a plug in a fermentor vessel which blew out when pressurized. Mother burned baby okay

11 FSHN Safety News Accidents that have happened while performing research in a lab Student using osmium to stain fat cells in a hood-didn t have hood turned on while working- could have caused blindness Student using trifluoroacetic acid for an assay. This was done in the hood as stated in the SOP; the hood was on but must have been faulty as the student inhaled some TFA fumes. Student complained a few days later of a sore throat and the next day he was coughing up blood and had to be hospitalized. PI found a different method! Seek an appropriate method that minimizes potential accidents

12 FSHN Safety News Accidents that could happened while performing research in a food lab Pressure pan/canner if lid is not secure or gasket needs to be replaced the lid can blow off and hurt someone Deep-fat frying watch oil temperature to prevent a fire As oil gets old the ignition point decreases so becomes more of a fire hazard dropping items into oil can cause spatters which can cause burns Steamers-hot vapors can cause burns Hot ovens Gas Ranges-open flames

13 FSHN Safety News New Online Safety Course -plan for spill response chemical biological radioactive

14 FSHN Safety News

15 FSHN Safety News First Aid Procedures 1. Move injured personnel only if necessary to prevent further harm. 2. For spills on small portions of skin, flush with water 15 min. If no burns, wash with soap & water. Remove jewelry to facilitate decontamination. 3. For spills on clothing or large portions of skin, initiate shower while removing clothing, shoes, and jewelry. Cut off clothing if necessary to not contaminate other parts of the body. 4. Neutralize spilled material if necessary (polyethylene glycol PEG300 for phenol exposure, calcium gluconate gel for hydrofluoric acid exposure) 5. For splashes into the eyes, irrigate the eyes for 15 min holding the eyelids away from the eyeball.

16 FSHN Safety News Seeking Medical Attention 1. Call 911 for medical emergencies. 2. SDS should accompany the person. 3. Notify the injured person s supervisor as soon as possible. 4. The supervisor is responsible for completing a First Report of Injury in AccessPlus.

17 FSHN Safety News Medical Treatment TYPE Life Threatening Injuries Non-Life Threatening Injuries After-Hours Treatment or Emergencies ACTION Call 911 to request an ambulance and notify supervisor as soon as possible. Administer immediate first aid using the First Aid Procedures. Report to supervisor, who will arrange medical care with McFarland Clinic, Occupational Medicine, P.C Duff Avenue, Ames, IA (515) Mary Greeley Medical Emergency Department (515) or 911, please notify staff when treatment is due to workplace injury or illness. Follow-Up Care McFarland Clinic, Occupational Medicine, P.C Duff Avenue, Ames, IA (515) Outside Ames Area Refer to the Human Resource Services list of Approved Medical Providers for treatment of work-related injuries.

18 Reporting FSHN Safety News All accidents and injuries occurring at work or in the course of employment must be reported to the employee's supervisor as soon as possible (even if no medical attention is required) and in the following ways: REPORT BY WHOM DIRECTIONS First Report of Injury (FROI) Supervisor or injured employee (reviewed by supervisor) Within 24 hours via AccessPlus - See next slide. Contact Environmental Health and Safety at (515) for guidance and assistance, especially when a serious injury or major loss occurs. Accident Investigation Incidents Resulting in Hospitalization or Fatality Supervisor Supervisor As part of FROI, be prepared to provide details about the incident. Supervisors are encouraged to obtain written accounts of the accident. Within 8 hours - call University Human Resources (515) and Environmental Health and Safety (515)

19 FSHN Safety News First Report Of Injury (FROI) Form 1.Logon to AccessPlus 2.Select Employee tab upper right hand corner 3.Select Work Injury on left hand side panel 4.Select Continue in middle of screen 5.Select the first line: Submit a new First Report of Injury 6.Determine if the injury requires just first aid or required more attention 7.Answer the questions as they appear on the screen The supervisor is responsible for completing a First Report of Injury (FROI) and submitting this electronically via AccessPlus within 24 hours of when the incident is reported. Employees can complete FROI's but supervisors must review, approve and electronically submit to University Human Resources.

20 FSHN Safety News First Report Of Injury Form Employee info Accident investigation Nature of injury Date and time of injury Parts of body affected Events that caused the injury Object/substance that caused the injury Activity the employee was engaged in when injury occurred Witness info Medical attention required Physician info

21 FSHN Safety Reminder Heating Devices Unplug when not using Hotplates malfunction Controls shorting and causing fires Use an automatic temperature controller for unattended operations Consider alternatives to mineral oil bath (flash point lowers over time) silicone oil, sand, metal beads, reaction blocks

22 FSHN Safety Reminder Use of Dust Masks/Resperators -if use is mandatory must be certified to use mask -medical review -annual training and fit test -if use is voluntary -certification not required -set of rules for use, storage and disposal -use only for purposes masks were designed for

23 FSHN Safety Reminder Biosafety cabinets should be used when performing -manipulations of airborne transmitted pathogens -manipulations of human blood which may contain pathogens -anything that might create aerosols of potential pathogens -vortexing, opening cap after centrifuging, sonicating, aspirating with syringe

24 FSHN Safety Reminder Water baths -fill with 1 mohm water -18 mohm is too pure and will pull the minerals out of the stainless steel. -water baths will look rusty

25 FSHN Safety Reminder Eye wash stations must be rinsed once a month Must be documented -blue tag -Excel spreadsheet

26 FSHN Safety Reminder Sharps MUST be placed in a puncture resistant container not trash Needles Pipet tips Plastic pipets If needle content is not known, tests will be done to determine needle content-pi will be charged for content identification!

27

28 Question 1 What are the 6 items of safety information that the FSHN department says should be kept near your Laboratory Safety Manual (LSM)?

29 Answers to Question 1 Standard Operating Procedures (SOPs) SDS Laboratory Chemical Inventory Personnel safety training records Yearly lab inspection sheets EH&S Lab inspection audits (has library of SOPs)

30 Question 2 What are the top 8 things that EH&S said you shouldn t do in a lab? pumpkinpie59.glogster.com

31 Answers to Question 2 Mouth pipette Eat Drink Chew gum Apply chapstick or makeup Chew on pencils/pens Touch lips with gloved or unwashed hands Taste chemicals

32 Question 3 What 5 pieces of information did EH&S say should be put on labels of secondary chemical containers??

33 Answers to Question 3 Chemical names in English Concentration Solution chemical is in Initials Date prepared or opened Signal word (flammable liquid) or NFPA symbol;

34 Question 4 What 7 safety items did EH&S say should be worn in the laboratory? What s wrong here?

35 Answers to Question 4 Safety glasses/goggles Lab Coat Gloves resistant to the material used Fully enclosed shoes Hearing protection Long pants Apron/face shield

36 Question 5 What 9 pieces of safety equipment in good working order did EH&S say all ISU laboratories should have?

37 Answers to Question 5 Hood/Biosafety cabinet Shower Eyewash station Chemical/Biohazard Spill kit Laboratory Safety Manual First aid kit Telephone with 911 stickers Fire extinguisher Flammable storage Ref/Freezers

38 Question 6 What 8 hazards did EH&S say that safety glasses, goggles and face shields protect your eyes from?

39 Answers to Question 6 Acids/Bases Organic Solvents Liquids under pressure (HPLC) Biological fluids Flying projectiles Ultra Violet light Βeta radiation protection

40 Question 7 What should you do if there is a fire in your laboratory?

41 Answers to Question 7 Turn off gas If gas is on Pull fire alarm Call 911 Assist injured personnel Evacuate all personnel Meet in prearranged area Use Fire extinguisher ONLY if the fire is small user must be trained extinguisher lasts only 6-8 seconds

42 Question 8 What 3 pieces of equipment did EH&S say must be used for working with or storing flammable solvents? What s wrong here?

43 Answers to Question 8 Flammable storage cabinet For closed containers Fume hood For active lab activities Explosion proof refrigerator/freezer For cold storage

44 FSHN Accidents Normal lab procedures -unattended use of stir plate for overnight dialysis of samples to remove salts -storage of samples until paper is published-peroxide forming chemicals in sample solvent

45 Question 9 What Top 10 items are on the EH&S list of lab violations in 2015 when performing a laboratory safety survey?

46 Answers to Question 9 Top 10 ISU Safety Violations 1. No chemical/bio/rad inventory 2. Incorrect container label (usually this is that they are not labeling with full chemical names) 3. Eyewash & safety shower (not tested or inaccessible ) 4. No training records available or training not completed 5. No emergency action plan (or plan has outdated contact information) 6. Electrical safety issues (primarily improper use of extension cords and/or power strips) 7. Satellite accumulation area issues (top 3 SAA problems are: 1. Waste not labeled 2. No cap on the container 3. Over 90 days) 8. Signage issues 9. Safety surveys not completed by lab staff 10. PPE not available, not used or used incorrectly

47 Question 10 What are the top 5 things EH&S said You should not do when working in a fume hood or biosafety cabinet?

48 Answers to Question 10 Forget to turn on power to the hood Store solvents Block back vents Block airflow on front edge or working surface Work with UV lights on cs/research/fumehood.jpg&imgrefurl= &h=378&w=393&sz=49&hl=en&start=54&tbnid=g4n9elm8hyprjm:&tbnh=119&t bnw=124&prev=/images%3fq%3dfume%2bhood%26start%3d40%26gbv%3d2%26n dsp%3d20%26svnum%3d10%26hl%3den%26sa%3dn

49 Question 11 What are 10 things EH&S said you should do for general lab safety?

50 Answers to Question 11 Lock the doors when unoccupied Question unknown persons Ask unauthorized persons (intruders) to leave. If need be call DPS (4-4428) Keep corridor doors (fire doors) closed Avoid working alone Keep work areas clean, uncluttered and aisles unobstructed Don t use ear buds or head phones- use devices with speakers at moderate level Confine long hair & loose clothing Food not for human consumption labels for research foods Sink hoses cut off above rim unless faucet has back flow prevention device Liquid hand soap and towels available at hand sink Wash hands after using chemicals and before leaving the lab Food, drink, gum, medications, cosmetics are not allowed labs Gas cylinders must be secured

51 Question 12 What should your response be to a lab accident?

52 Answers to Question 12 Determine what First Aid is needed Attend to hurt person Dial 911-identify yourself and location-stay on phone until told to hang up Stay with person until medical attention has arrived Notify supervisor of injured party Supervisor must fill out First Report of Injury within 24 hours

53 FSHN Safety News We encourage the faculty, staff, and students to be diligent about safety in their labs at all times. Thank you!

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