NPDN: A Record of Accomplishments

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Conrad Payne
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1 NPDN: A Record of Accomplishments A Consortium of Regional Networks Working together to protect U.S. agriculture

2 Network Mission To enhance national agricultural security by rapidly detecting introduced pests and pathogens. Educate to Detect First detector training and registry Increased awareness Diagnose Training and support for diagnostic infrastructure National standards Surge capacity Communicate Exercise scenarios Secure communications Coordination and cooperation among agencies Information to assist response of regulatory agencies Harmonized reporting protocols Secure national data repository cataloguing pest and disease occurrences Newsletters and public relations

3 Information technology committee Diagnostics committee Training and education committee National database committee NPDN Operations Committee Website committee Epidemiology committee Exercise committee Public relations committee

4 Interagency Partnerships Land Grant Universities Federal Agencies State Departments of Agriculture

5 North Central Plant Diagnostic Network Michigan State University North Eastern Plant Diagnostic Network Cornell University Western Plant Diagnostic Network University of California, Davis Including Alaska, Hawaii, & US territories Great Plains Diagnostic Network Kansas State University CERIS Purdue University Southern Plant Diagnostic Network University of Florida Including Puerto Rico

NPDN: A Record of Accomplishments Education and Training First Detector and FD

hereby granted Tom Jefferson for completion of core First Detector and training

Date: 17 th August 2006 Location: Las Vegas, NV This certificate has been approved

Education Ri cha r d Hoen i sch Training Session Coordinator First Detector

2: Monitoring for High Risk Pests Module 3: Quality and Secure Sample Submission

6 NPDN: A Record of Accomplishments Education and Training First Detector and FD Educators: modules and manuals Certificate of First Detector Training Completion is hereby granted Tom Jefferson for completion of core First Detector and training modules. Date: 17 th August 2006 Location: Las Vegas, NV This certificate has been approved by Kitty Cardwell, PhD, NPDN Chair and Gail Wisler, PhD, Chair of NPDN Training and Education Ri cha r d Hoen i sch Training Session Coordinator First Detector Certification Module 1: The Mission of the NPDN and Agricultural Biosecurity Module 2: Monitoring for High Risk Pests Module 3: Quality and Secure Sample Submission Module 4: The Art and Science of Diagnosis: Plant Pathogens and Arthropods Module 5: First Detector Exercises Module 6: Effective Photography for Digital Sample Submission

7 NPDN: A Record of Accomplishments Training and Education over 300 basic awareness training sessions reaching > 12,000 people nationwide approx. 6,000 First Detectors registered with NPDN

8 NPDN: A Record of Accomplishments Education and Training Diagnosticians County Extension Educators/Agents Ag Commissioners and staff Farm Advisors First Detectors Growers Nursery Producers Crop Consultants Ag Biologists Cooperative extension personnel Pesticide applicators Master Gardeners Commercial seed representatives Conservation biologists

9 NPDN: A Record of Accomplishments Education and Training Next generation tools for National Training in Crop Biosecurity for First Detectors USDA-NRI project: G. Holmes (NCSU), H. Beck (UF), & G. Snyder (KSU) 1. Converting NPDN instructor-led training modules into online training modules 2. Database - driven technology: beyond Powerpoint Lyra

10 NPDN: A Record of Accomplishments Education and Training Regional Pest Alerts in collaboration with national IPM centers

11 NPDN: A Record of Accomplishments Education and Training Entomology and Insect Vector workshops Florida, Hawaii, California, Montana, Virginia Coleoptera, Homoptera, thrips, pink hibiscus mealybug

12 NPDN: A Record of Accomplishments Education and Training Sudden Oak Death/Ramorum blight a one day national on-line training teleconference held on October 26, 2004 Coordinated by National IPM Centers, NPDN, and USFS USDA Phytophthora ramorum Educate to Detect (PRED) Program over 700 participated Rhododendron leaf blight

13 NPDN: A Record of Accomplishments Education and Diagnostics Specialized training for diagnosticians USDA-Beltsville PCR workshop in Amarillo

14 NPDN: A Record of Accomplishments Diagnostics Diagnostic resources and support Real-time PCR Web-enabled microscopy And training with APHIS PPQ CPHST on SOD/Ramorum blight and citrus greening agent Collaborative diagnostics

15 Distance diagnostics

16 NPDN: A Record of Accomplishments Diagnostics SOP s eight (and counting) for significant agents Coordination with APHIS PPQ of the permitting process for NPDN labs

17 Diagnostics - Success Stories Ralstonia solanacearum race 3 biovar 2 Asian soybean rust/ Phakopsora pachyrhizi Sudden Oak Death/Ramorum blight Plum Pox virus

Ralstonia Bacterial Wilt Ralstonia solanacearum race 3 biovar 2. Pathogenic on: Geranium Bananas Pepper, tomato, eggplant, potato 2003-4: several introductions into the U.S.

18 Ralstonia Bacterial Wilt Ralstonia solanacearum race 3 biovar 2. Pathogenic on: Geranium Bananas Pepper, tomato, eggplant, potato : several introductions into the U.S. (holds on 489 nurseries across the country) Occurred in geranium cuttings from production greenhouses outside the U.S.; holds on hundreds of nurseries across the country) Concern for carry-over to potatoes and other solanaceous crops and weeds. NPDN labs processed samples and helped validate protocols. courtesy Natalie Goldberg

2000 - the pathogen was confirmed to be P.

19 Sudden Oak Death Ramorum Blight Phytophthora ramorum First identified in 1993 in Europe on Rhododendron and Viburnum a new disease killing tanoak and oak trees observed in California the pathogen was confirmed to be P. ramorum. Natural infections limited to Europe, California and Oregon. Problem in plant nurseries!

20 Sudden Oak Death Ramorum Blight Photos: Jonathan Jones, APHIS-PPQ; Jennifer Parke, Oregon State Univ.

21 SOD/Ramorum blight trace-forwards and positive detections July 2004 Map: USDA, APHIS, PPQ NPDN labs processed over 110,000 samples Provisional Laboratory Certification

Soybean rust /Phakopsora pachyrhizi 2004 and beyond Nov 6

and local APHIS begin distance diagnostics process with

8 sample in system for confirmatory diagnosis with APHIS;

general NPDN release Nov 9 APHIS alerted and PCR confirms

22 Soybean rust /Phakopsora pachyrhizi 2004 and beyond Nov 6 LSU researcher notes suspect sample in soybean plot; LSU and local APHIS begin distance diagnostics process with national APHIS. Nov. 8 sample in system for confirmatory diagnosis with APHIS; LSU notifies SPDN of possible SBR in US not for public or general NPDN release Nov 9 APHIS alerted and PCR confirms Nov 10 USDA/APHIS/Secretary of Ag releases information to public Nov 11/18 - NPDN conference calls on SBR

NPDN: A Record of Accomplishments Asian Soybean Rust Team 2006 Secretary s Honor Award advance planning, rapid response and recovery of a disaster that was

24 NPDN: A Record of Accomplishments Asian Soybean Rust Team 2006 Secretary s Honor Award advance planning, rapid response and recovery of a disaster that was averted by using science-based and technological strategies or addressing and mitigating risks for a potentially devastating plant disease, the Asian Soybean Rust.

25 Citrus greening/liberobacter spp. Recent Pests of Note Pink hibiscus mealy bug Maconellicoccus hirsutus Potato cyst nematode Globodera pallida Erythrina gall wasp Asian longhorned beetle

26 NPDN: A Record of Accomplishments Communication Exercise Program NPDN Notification Presumptive Positive Producer First Detector CPHST NIS or SEL National Program Administrator NPDN Diagnostician (May be LGU Or SDA) SPRO SPHD NPDN Regional Director APHIS Eastern or Western Region NPDN National Program Leader (CSREES)

Exercise Program Soybean rust, Ralstonia, SOD, Oleander

woodwasp, ALB, glassy winged sharpshooter Partnering

US-Mexico Border Governor s Agriculture Worktable

27 Exercise Program Soybean rust, Ralstonia, SOD, Oleander scorch (Xylella), banana sigatoka, potato wart, Sirex woodwasp, ALB, glassy winged sharpshooter Partnering with APHIS, DHS Office of Domestic Preparedness, NAHLN, US-Mexico Border Governor s Agriculture Worktable exercise NPDN - APHIS full-scale exercises in Texas and Colorado

29 NPDN: A Record of Accomplishments National Database Committee January 2006 Communication Information Technology Regional and National Efforts Management and analysis of diagnostic lab data at local, regional and national levels Secure national integrated research data repository Harmonized reporting protocols Accommodates state practices and protocols Quality control at multiple levels plans to automate Efficient query and analytical tools How and what diagnostic data should be delivered

30 NPDN: A Record of Accomplishments Communication automated delivery of data with PDIS, DDDI Approx. 140,000 sample records in National Data Repository

e-mail with the addition of encryption, security,

31 NPDN: A Record of Accomplishments Communication Secure communications Provides functionality of with the addition of encryption, security, tracking, secure archiving, and increased size capability

32 NPDN Acronym Translator Type letters here NPLB Your program/agency answer: No Plant Left Behind suggested logo

33 NPDN: A Record of Accomplishments Communication Epidemiology Goal: design, develop and implement analyses using the NPDN data repository and other databases Purpose: To detect outbreaks at the earliest stage possible Outbreaks may be local, state, regional or national events.

35 NPDN: A Record of Accomplishments Communication Epidemiology Integrated GIS Platforms Distributed Integrated Interfaced National Database Managed locally, distributed nationally

38 NPDN: A Record of Accomplishments Communication Website, Public Relations, Newsletter

39 Plant Disease (2006) 90:

40 NPDN Continuing goals 1. Coordination and governance mechanisms are functioning for the regional and national networks. 2. Educated/capable first detectors. 3. Data systems and network integration are functioning. 4. Functioning diagnostic processes. 5. Data analysis processes for event detection are functioning. 6. Response activation and decision support mechanisms are functioning. 7. Sustainable funding is achieved.

41 Additional NPDN revenue streams

42 NPDN: A Record of Accomplishments The history of NPDN is a record of accomplishment through effective and strategic partnerships. The most important resource to ensure success in any endeavor is people. NPDN has benefited from the talents of a long list of dedicated and knowledgeable individuals working together as a highly efficient team. Without those individuals, this network would not be successful.

The National Plant Diagnostic Network Standard Operating Procedure for: APHIS-PPQ Pest of Concern Scenario General SOP. ** rev May 7, 2010

The National Plant Diagnostic Network Standard Operating Procedure for: APHIS-PPQ Pest of Concern Scenario General SOP ** rev May 7, 2010 Table of Contents Step 1: Sample Submission..Page 2 Step 2: Sample