Five Day Advanced Survey HAPSITE SMART Operators Course
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1 Five Day Advanced Survey HAPSITE SMART Operators Course I. Day One a. Welcome and Introductions b. Use and limitations of HAPSITE c. Review of current FY PAT testing objectives i. Basic vs. Advanced skills ii. How grading works iii. Up-load procedure preview for PAT round data iv. Who needs to participate vs. who can d. Break e. Gas Chromatography / Mass Spectroscopy (GC/MS) Overview i. GC Operation principles ii. MS Operation Principles f. HAPSITE Components Review i. How is a sample drawn in 1. Probe 2. Headspace ii. Configuration i.e. Sample Loop vs. Concentrator 1. Use February 2008 epac: The Concentrator 2. Types of concentrator 3. V-G conversion tube introduction iii. MS Manifold Review (Have Demo Parts of each listed below(and the whole manifold)) 1. NEG Pump Review 2. Ion Pump 3. Ionizer 4. Quadruple 5. Electron Multiplier iv. A look inside v. Service Module 1. How used i. When ii. What does it do 2. Necessary repairs i. Part Replacement within manifold ii. NEG activations
2 (Day One Continued) g. Lunch h. Tuning i. Auto-tuning ii. Results 1. Front Panel of instrument 2. Tune Text report generation from the PC iii. Manual Tune demonstration by the instructor iv. Analytical Section members attending this course will be given a 1 on 1 tutorial with their instrument during PMCS completion if their ALS is not upgraded and still has a HAPSITE i. JPMESG Method Introduction i. Method nomenclature ii. CST Standardization (No creation of method) Operators need to use and be limited to validated methods iii. Quality Control (Methods are only valid if QA/QC procedures are followed) iv. What method should you choose 1. PID / CAMS data 2. Always mssurvey first 3. Method sensitivity chart v. March and May 2008 epacs: Method Selection I and II vi. Method selection exercise j. Break k. PMCS i. Why is it necessary ii. Completion 1. Required Materials i. Performance Standard i. Storage and use ii. Preparation: Appendix 1 to JPMESG SOP gc_cbqc and gc_txqc ii. Concentrator type and installation i. February 2008 epac: The Concentrator ii. What could go wrong 1. Broken Concentrator
3 (Day One Continued) 2. How to maintain the instrument after a breakage iii. 10uL Syringe care and use techniques iii. August 2007 epac: PMCS 1. Read epac 2. Complete challenge which is to perform a PMCS l. Website Introduction i. Logging in / Signing up ii. Uploading PMCS results iii. Reporting a Problem iv. Reviewing FAQs v. Reviewing previous logged calls vi. Download Center m. NGB Reach back/maintenance Procedures i. Nationwide Business Hour Phone Technical Support by Support Center SMEs ii. Support Center Equipment Repair/Exchange Procedures 1. DSO Rep /2765 Forms II. Day 2 a. Review of Day 1 material (Quiz) b. Use Limitations and Theory i. Why the HAPSITE is necessary 1. Trace level detection not capable by other instruments 2. Low level CWA air monitoring ii. Detection limitations for CWAs iii. Comparison of capabilities between HAPSITE and other fielded Survey equipment iv. How do we detect nerve agents? c. Use of mssurvey i. Slide set to explain the differences between mssurvey and full-scan methods ii. Always use mssurvey to screen samples iii. Comparison between mssurvey use and detection limits vs. other fielded equipment d. Qualitative Data Interpretation (Advanced HAPSITE Operator Skills) i. Four Steps
4 (Day Two Continued) 1. Automated Forward NIST search i. Examples with Operator interaction on Computer ii. Evaluating peaks for use in the search iii. How to print the report and export electronic data for use in PAT reporting 2. Manual Forward NIST Search i. Examples with Operator interaction on Computer ii. Find Sarin in the provided Chromatogram 3. Automated Reverse Search Using AMDIS i. Examples with Operator interaction on Computer ii. How to print the report and export electronic data for use in PAT reporting 4. Use of RICs i. Separating Co-eluted peaks ii. How to find ions of interest for suspected components in a sample iii. Confirming or Denying the presence of CWAs e. Break f. September 2007 epac: Qualitative Data Interpretation g. Lunch h. Example CWA interpretation Exercises i. Break j. PMCS completion k. mssurvey scenario exercise III. Day 3 a. Review of Day 2 material b. Qualitative vs. Quantitative Data Interpretation i. When and Why to use ii. Benefits of Quantitative Analysis 1. GC/MS Dual Confirmation (100% GC/MS ID) 2. Concentration from a calibration curve vs. Estimation c. Break d. In depth Quantitative data interpretation i. Production of the Unknown Identification Report (UIR)
5 (Day Three Continued) ii. Interpretation of the UIR iii. Reporting the concentration range from the UIR iv. Completion of the August 2008 epac: Unknown Identification Report v. Estimating concentrations for compounds not found in the UIR 1. Always use Qualitative interpretation first 2. Estimation using areas 3. Estimation using TIC information 4. Estimation of concentration for Co-eluted compounds vi. Completion of the September 2008 epac: Estimation of Concentration e. Lunch f. Quantitative data analysis scenario exercises using CWA chromatograms g. Break h. PMCS Completion IV. Day 4 a. Review of Day 3 Material b. PMCS Completion for Sample analysis c. Break d. NGB PAT Round Program Overview i. Why it is required (ISO Team Certification) ii. Who is required to participate? iii. How to sign up iv. Proper submission of results to CST Data Net website e. Simulated PAT Round completion i. Reading and interpreting information from first responders ii. Selection of the Proper Method iii. Data collection and analysis iv. How to submit results f. Lunch g. Completion of Additional Simulated PAT Round sample h. Break anytime during PAT analysis i. Completion of TIC scenario exercise (time Permitting)
6 V. Day 5 a. Final written examination b. Break c. Analysis of Simulated PAT Round Sample i. Passing of Final Exam ; requires successful completion of PAT round unassisted. ii. No instructor assistance except that which can be received utilizing NGB Support Center Reach-back d. Lunch anytime during analysis e. Each student or group of students will review results with the instructor prior to being released
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