2008 AZA CONSERVATION ENDOWMENT FUND Cover Sheet

Transcription

1 2008 AZA CONSERVATION ENDOWMENT FUND Cover Sheet Title of Project: Oregon Spotted Frog Recovery Project Amount Requested: $29,956 (round to the nearest whole dollar) Project Number: For AZA use only Principal Investigators: Each Principal Investigator (PI) must be an individual AZA member at the time of application. Institutional membership does not count as individual membership. Other participants should be listed as Collaborators in Question 12 on this form. To apply for membership, visit A Curriculum Vitae (CV) is required for each PI, and a Statement of Institutional Support (SIS) is required for each PI Institution. See the CEF Application Instructions for important formatting instructions. These instructions and the SIS form are available on the AZA website: 1. Principal Investigator (PI) Name and Title: Lisa Dabek CV Attached? (required no more than 2 pages) Individual AZA Member Number: REMOVED (required) Institution/Organization: Woodland Park Zoo Statement of Institutional Support Form Attached? (required) Institutional Address: REMOVED Phone: REMOVED Fax: REMOVED REMOVED 2. Principal Investigator (PI) Name and Title: Dana Payne CV Attached? (required no more than 2 pages) Individual AZA Member Number: REMOVED (required) Institution/Organization: Woodland Park Zoo Statement of Institutional Support Form Attached? (required) Institutional Address: REMOVED Phone: REMOVED Fax: REMOVED REMOVED 3. Principal Investigator (PI) Name and Title: David Shepherdson CV Attached? (required no more than 2 pages) Individual AZA Member Number: REMOVED (required) Institution/Organization: Oregon Zoo Statement of Institutional Support Form Attached? (required) Institutional Address: REMOVED Phone: REMOVED Fax: REMOVED ** If you need to add additional Principal Investigators, please add their information in a separate Word document titled Additional PI and include it as an attachment to your CEF Application .

2 Project Categories (Choose Two): Management/Captive Breeding (Ex situ) Conservation Education Field Conservation (In situ) Research Animal Health Animal Welfare Amphibian Fund interest: For amphibian-related projects only: I/we have read the AZA Amphibian Fund Guidelines (available at and would like this proposal to be considered for support from the Amphibian Fund. Yes No Project Abstract (Limit 1,500 Characters with spaces): Once common, Oregon spotted frogs were thought to have been extirpated from Washington State in the 1960s, until an isolated population was found in Thurston County in They are classified as endangered in Washington state, and they are a strong candidate for listing under the Federal Endangered Species Act. In 2007, Oregon Zoo, Northwest Trek, and Woodland Park Zoo, Washington Department of Fish and Wildlife and Ft. Lewis Fish and Wildlife Program came together to form the Oregon Spotted Frog Recovery Project. The ultimate goal of the project is to increase wild populations of Oregon spotted frog by reintroducing frog metamorphs into federally protected habitat in Washington state. A small-scale pilot phase of this project is underway. Woodland Park Zoo has been asked to help expand the overall capacity of this effort by building a full-scale headstarting facility and acquiring the equipment necessary to contribute to efforts to headstart the frog. In addition, WDFW has requested Woodland Park Zoo s assistance in drafting an updated Recovery Plan. We are requesting $29,956 from CEF to help reach these goals. CEF funds would cover some of the costs of materials to construct a headstarting facility at Woodland Park Zoo, cover costs associated with transporting metamporphs to the release site, and cover the stipend of a student research technician who will assist the Washington Department of Fish and Wildlife in finalizing an updated Recovery Plan for the frog.

3 Proposal Instructions: 2008 AZA CONSERVATION ENDOWMENT FUND Application Form Instructions Project Proposal: Answer Questions 1-12 by typing in the form fields in the Project Proposal section below (gray boxes). Form fields have a limit on the number of characters that may be used in order to keep the application concise. Character limits are stated following each question. The stated limits are for characters with spaces. If a question is not applicable to your proposal, write N/A in the field. Do not leave any questions blank, as this may delay processing of your application. Note that spell checker and track changes functions cannot be used in this form. AZA recommends that applicants compose their Project Proposal in a separate document if they wish to make use of these features, and copy and paste the final text into the form fields below. When composing in a separate document, be aware of character limitations. If the copied text exceeds the character limit when pasted into a form field, all text exceeding the character limitation will be cut off and will not appear in the final proposal. Also note that certain formatting such as italics, bold, and underlining, is unavailable due to the application format. AZA recommends the use of numbering and spacing to emphasize important points in the text. Project Budget: Detailed budget instructions can be found in the Project Budget section of the application. Required budgetary information includes: 1) Information regarding previous CEF support received by any PI listed on the Cover Sheet. 2) Total Project Budget and amount requested from CEF. Each should be for one year. Other financial support received or applied for should be listed as well. 3) Detailed Budget listing specific project items and funding source for each. 4) Budget Justification explaining why major items are essential to the project and reasonable in cost. Only complete applications can be considered for review and possible funding. Applicants will be informed in writing if their application is incomplete. Application Submission: Applications must be submitted electronically. Hard copies of any application materials will NOT be accepted. Applications and supplemental materials must be submitted as attachments in a single CEF Application to cefapp@aza.org. Applications must be received in an with your proposal title as the subject line. If your title is too long, please include as much as you can in the subject line. Completed applications must be received via in the CEF Applications Inbox (cefapp@aza.org) no later than 5:00 p.m. EDT on 4 April Any s time-stamped later than this will not be accepted. For important additional information regarding application submission and more detailed instructions, see the CEF Application Instructions, available at Application Assistance: Proposals received at least one week prior to the application deadline will be reviewed for adherence to CEF guidelines and will be returned for resubmission if problems are noted. Please note that AZA staff will not review early proposals for content. The CEF website also contains a wealth of information to assist you in completing this proposal. Please review the resources at You may also contact the AZA office for assistance at cef@aza.org or **If you d like to include citations/references for statements made in the Project Proposal, please include them in a separate Word document titled Citations and attach it to your CEF Application . Citations are NOT required. **If you d like to include additional appendices, please include them in a separate Word document titled Appendix and attach it to your CEF Application . Be sure that any appendices follow the guidelines stated in the CEF Application Instructions. Appendices are NOT required.

4 2008 AZA CONSERVATION ENDOWMENT FUND Project Proposal 1. Explain the goal(s) and rationale behind the proposed project. What will the specific outcomes be? (If the proposal is for funding part of a larger project, the proposal must focus on the part for which CEF funding is being requested.) LIMIT 4,000 CHARACTERS with spaces Rationale: The Oregon spotted frog (Rana pretiosa) is a medium-sized frog, growing to a maximum body length of about four inches. It belongs to the same family as bullfrogs, leopard frogs and red-legged frogs. Even more aquatic than their more familiar cousins, they are usually found in or very near water. In spite of their name, they once ranged from southwestern BC to northwestern California. Oregon spotted frogs were thought to have been extirpated from Washington State in the 1960s, until an isolated population was found in Thurston County in Subsequently, three additional populations were located in southern Washington State. Ten years after the Washington Department of Fish and Wildlife s most recent Management Report classified them as a State Endangered Species, the Oregon spotted frog remains endangered in Washington. This species is a candidate for listing under the Federal Endangered Species Act and has been classified as a Species of Greatest Conservation Need in the 2005 Comprehensive Wildlife Conservation Strategy (CWCS) prepared by the Washington Department of Fish and Wildlife (pp. 172, 621). As cited by CWCS, the Oregon spotted frog is highly threatened and highly vulnerable, remains endangered, is inadequately funded and only partially protected (pp.621, ). Increasing the distribution and abundance of populations in western Washington is a recognized recovery strategy for the Oregon spotted frog. Additional populations will help reduce the vulnerability of the species to catastrophic losses. Therefore the ultimate goal of the Oregon Spotted Frog Recovery Project is to reintroduce frog metamorphs into westside riparian and herbaceous wetlands federally protected habitat identified and protected as priority conservation actions in the Puget Trough ecoregion (CWCS, p.172). The pilot phase of the Oregon Spotted Frog Recovery Project began in summer, Participants, including Oregon Zoo, Northwest Trek and Woodland Park Zoo, came together with the Washington State Department of Fish and Wildlife to plan recovery efforts for the species. Since then, Oregon Zoo (Portland, OR) and Northwest Trek (Eatonville, WA), have set up pilot-phase headstart facilities using protocols developed by Mountain View Conservation & Breeding Center. Eggs were collected from sites in Washington state, and tadpoles are currently being headstarted at the participating zoos. Approximately 240 metamorphs are expected to be released in suitable habitat at Ft. Lewis Military base in September, Goals and Outcomes Specific to This Request: Woodland Park Zoo has been asked to help expand the overall capacity of this effort by building a full-scale headstarting facility and acquiring the equipment necessary to contribute to the headstarting effort in the 2009 season and beyond. In addition, WDFW has requested Woodland Park Zoo s assistance in drafting an updated Recovery Plan for the frog. We are requesting $29,956 from CEF to help reach these goals. CEF funds would cover the costs of materials to construct a headstarting facility at Woodland Park Zoo, cover costs associated with transporting metamporphs to the release site, and cover the stipend of a student research technician who will assist the Washington Department of Fish and Wildlife in finalizing an updated Recovery Plan for the frog. Please note: although the "pilot phase" of Oregon spotted frog headstarting is already underway as noted above, none of the expenses for which we are requesting CEF support will be incurred before September Specific outcomes of CEF funding will include 1) Scaling up the Oregon Spotted Frog Recovery Project, increasing total Project capacity more than 10 times over 2) Completing an up-to-date Recovery Plan for the Oregon spotted frog and 3) Releasing more than 3,000 Oregon spotted frog metamorphs back into the wild in September, 2009.

5 2. What is the conservation/management significance of the specific outcomes? Why do you consider the project to be timely? How does it address a current critical need? Explain how the project is compatible with the purposes of the CEF, as stated at LIMIT 4,000 CHARACTERS with spaces An unprecedented crisis currently threatens amphibians around the world. According to the World Conservation Union s Global Amphibian Assessment published in 2004, the 6,000 species of frogs, salamanders and caecilians known worldwide are experiencing dramatic declines in population with one third to one half of all amphibian species facing possible extinction. Scientists estimate that since 1980 at least 122 species had already gone extinct. For years, amphibian populations have been under the pressures of habitat loss, pollution, and climate change and now face a new and deadly challenge, the disease chytridiomycosis. As detailed in the Amphibian Taxon Advisory Group s Regional Response Plan, AZA zoos and aquariums are called upon to respond to this crisis. In addition to exposing visitors to exotic species from around the world, zoos must act on behalf of the endangered species in their own backyards.woodland Park Zoo, Oregon Zoo and Northwest Trek have determined our organizations are especially well positioned to assist with recovery efforts for the endangered Oregon spotted frog. Washington Department of Fish and Wildlife considers this species a high priority for conservation and has enlisted our organizations to assist them in their efforts to restore the frog to its native habitat. This effort is especially timely, because 2008 has been designated Year of the Frog by the AZA and is thus an auspicious time to begin an ex-situ program to help the frog. This will be a valuable new species recovery program for the recently-formed Northwest Zoo and Aquarium, part of the regionally-based conservation efforts for AZA institutions. In addition to giving our organizations a compelling story with which to communicate our central conservation message to our audiences, the Oregon Spotted Frog Recovery Project represents a significant contribution to the conservation of native amphibians in Washington with implications for other efforts in the Pacific Northwest and around the world. This project is in direct alignment with purposes of CEF because it 1) reflects the priorities of the Amphibian Taxon Advisory Group s Declining Amphibian Regional Response Plan, which proposes that AZA institutions commit to saving at least one local endangered species of frog 2) represents a timely response to AZA priorities, in so far as AZA has declared 2008 to be the Year of the Frog 3) provides a direct link between zoo frog collections and the conservation of endangered or threatened wildlife and ecosystems in North America 4) implements a conservation goal, to return the endangered Oregon Spotted Frog to a portion of its native habitat 5) is collaborative in nature, bringing together Woodland Park Zoo, Oregon Zoo, and NW Trek with the Washington Department of Fish and Wildlife and Ft. Lewis Military base to meet a common goal 6) shows high potential return on investment with direct conservation impacts measurable within one year 7) is feasible and fiscally and scientifically sound, employing proven methodology developed by Mountain View Conservation & Breeding Center in British Columbia, Canada and 8) stands to benefit a large number of animals by establishing a precedent for other frog conservation efforts in North America and around the world. 3. Explain the methodology for this project and why it is appropriate. (For instance, if this is a research project, clearly state the hypotheses to be tested; if this is a conservation education or professional training project, describe your target audience and how many people will be reached, etc.) LIMIT 6,500 CHARACTERS with spaces The methodology for this project will consist of three main activities: headstarting immature frogs, monitoring frog populations in the wild, and updating the Oregon Spotted Frog Recovery Plan. Headstarting: To conduct headstarting at Woodland Park Zoo, we will need to first construct the appropriate facility. This work will begin in September A framework covered with chain link will enclose the headstarting space, which is open to the weather. Inside, heavy-duty Rubbermaid tubs fitted with specially-made lids will house the tadpoles and justmetamorphosed frogs. A plastic cistern will be used to hold aged or treated water for routine water changes, and could be used to store collected rain water. In spring of 2009, the Washington Department of Fish and Wildlife will collect eggs from sites in the Black River drainage in Thurston County, Washington and Conboy Lake in Klickitat County, Oregon and will transport them to Woodland Park Zoo, Oregon Zoo and Northwest Trek. In order to maximize genetic diversity and minimize impact on extant populations, a sample of 20 eggs will be collected from each egg mass.

6 Eggs for headstarting will be transported to each participating zoo in large (quart-sized) plastic yogurt containers filled halfway with water and then stabilized in a larger container with Styrofoam or recycled peanuts. Jostling can damage eggs or cause premature hatching, so all care will be taken to minimize egg movement during transport. Eggs hatch between days after laying. At each partner zoo, tadpoles will be reared in aquariums on a diet of varied vegetation and commercial fish foods. This headstarting process will take place February 2009 through September Two hundred eggs will be collected to stock each tank; each tank is expected to result in 100 tadpoles. As frogs metamorphose, expected in fall of 2009, they will be screened for disease and transported to the Dailman Lake wetland located on the Fort Lewis Military Base for release. Prior to release, the site will be monitored for presence of predators and chytrid fungus. Monitoring: Pre-release monitoring will take place prior to release in September 2008, and again in late summer Activities will include testing for chytridiomycosis, to guard against releasing headstarted metamorphs into an area where the disease is present. Post-release monitoring of individuals released as part of the 2008 pilot season will be conducted throughout the fall of 2008 and will consist of regular surveys to determine the presence of headstarted metamorphs. Approximately 20 individuals will be affixed with transmitters, which will allow for radio tracking in late February and early March, Approximately 20 individuals will be affixed with radio transmitters prior to the 2009 release as well in anticipation of the 2010 field season. Egg nest searches will be added to monitoring activities in March, 2010 once the frogs released in September, 2008 have had an opportunity to mature. Updating the Recovery Plan: In addition to headstarting and monitoring efforts, creating an updated Recovery Plan for the Oregon spotted frog is a significant goal in the 2009 field season. Starting in September 2008, a qualified student technician will intern at Woodland Park Zoo s Conservation Department to finalize the Oregon Spotted Frog Recovery Plan. Working with staff at the Washington State Department of Fish and Wildlife, the student technician will first update the existing Management Report, created in The outcome will be a finalized Recovery Plan, completed no later than April, 2009 and will be used by all partners including state agencies, conservation organizations and AZA institutions, to guide Oregon spotted frog recovery activities. The methodology described above is appropriate because it follows successful protocols developed Mountain View, a privately-owned conservation center in Fort Langely, British Columbia. In 2007, Mountain View reported an overall success rate of 78% from hatch through morphing. The Ft. Lewis site, where the frogs will be reintroduced into the wild, is unique in the lower Puget Trough in that it is not subject to the same development pressures that exist outside of the base a uniquely protected location that will help ensure a successful reintroduction effort. 4. If you received previous CEF funding for this or a related project, please summarize relevant findings and how they relate to the current proposal. LIMIT 1,000 CHARACTERS with spaces N/A 5. Who are the personnel for this project, what are their roles, and what are their qualifications? Discuss each PI listed on the Cover Sheet, and any collaborators. A collaborator is defined as any person actively involved in the design or completion of the project. Each collaborator s institution should be listed in Question 10 on this form. LIMIT 2,500 CHARACTERS with spaces Principal Investigators: Lisa Dabek, Director of Conservation at Woodland Park Zoo, oversees all zoo-supported conservation programs. Dr. Dabek earned her Ph.D. in behavioral ecology (emphasis in conservation biology) at UW, Seattle, Dr. Dabek will supervise the student technician who will work with WDFW to update and finalize the Recovery Plan for the Oregon Spotted frog.

7 Dana Payne, Curator at Woodland Park Zoo, has been with the zoo since 1982 and has more than 27 years of experience caring for reptiles and amphibians in captivity. His experience with large scale amphibian breeding projects includes work with ranids, leptodactylids, and bufonids. He is on the steering committee for the Snake TAG, and is an AZA Antivenom Index advisor. Payne will be responsible for all spotted frog recovery efforts at Woodland Park Zoo and coordination with project partners. David Shepherdson, Deputy Conservation Division Manager at Oregon Zoo, holds a B.Sc. (Honors) in Biology and a Ph.D. in Ethology from the University of Sussex, UK. He has been with Oregon Zoo since 1991 and serves as Co-Chair of the Northwest of Zoos and Aquarium s Species Recovery Group for the Oregon spotted frog. Shepherdson will coordinate the involvement of the Oregon Zoo in the Oregon Spotted Frog Recovery Project. Dave Ellis, Deputy Director at Northwest Trek Wildlife Park, holds a BS Biology and Agriculture from Berry College in Rome, GA. He has been with Northwest Trek since 1984 and an AZA Professional Fellow since Ellis will be responsible for all spotted frog recovery efforts at Northwest Trek Wildlife Park and coordinating functions with all project partners. Collaborators: Harriet Allen, Endangered Species Program Section Manager, Washington Department of Fish and Wildlife, has worked as a scientist and manager with WDFW for 20+ years. Allen directs the writing of all Washington State Recovery Plans. She will work with the technician to create the updated plan for the Oregon spotted frog and ensure egg mass collection is coordinated. Jim Lynch, Fish and Wildlife Biologist with the Fort Lewis Fish and Wildlife Program, has a Master of Environmental Studies with thesis work on Western Toad Habitat Use and Distribution at Fort Lewis Military Reservation. Lynch has over 10 years of experience working with amphibians and is responsible for amphibian monitoring and recovery at the Fort Lewis release site. 6. Describe your evaluation plan. Explain how you will ensure that you are meeting the goals and objectives of your project. Include how your evaluation plan will measure program effectiveness and apply evaluation data to strengthen the project. If this project has an educational or professional training component, the educational impact should be discussed (ideally including impacts on conservation-related knowledge, attitudes/affect, and behavior). Evaluation plans may be quantitative or qualitative. LIMIT 3,000 CHARACTERS with spaces To evaluate this project the following measurable objectives will be tracked: number of eggs collected (goal is at least 10,000); percentage of embryos raised to metamorph by zoos for release (goal is at least 50%); number of metamorphs released at Fort Lewis (goal is at least 3,000). Over the long-term, the following metrics will determine the success of head starting efforts: number of adult frog sightings post-release at Fort Lewis; number of egg masses observed at Fort Lewis in subsequent years, (starting spring 2010, beyond the duration of proposed project); number of zoo and aquarium visitors exposed to project. 7. Will this project be sustainable beyond the life of the grant? If so, explain how. How and when will the information generated by this project be disseminated? LIMIT 1,500 CHARACTERS with spaces This project will be sustainable beyond the life of the grant, because once the materials have been acquired to start up headstarting efforts at Woodland Park Zoo and the Recovery Plan has been created, ongoing project costs will be relatively minimal. Woodland Park Zoo, Oregon Zoo and NW Trek are committed to participating in headstarting in future years as one aspect of our contribution towards international efforts to halt the global amphibian crisis. The Oregon Spotted Frog Recovery Plan, to be completed as part of this project, will be distributed by the Washington Department of Fish and Wildlife. The Plan will be available to the public on the WDFW Web site by September, If this is a multi-year project, what is the funding strategy for subsequent years?

8 LIMIT 750 CHARACTERS with spaces Any ongoing costs of this project including staff time and equipment replacement will be assumed by Woodland Park Zoo, Oregon Zoo and Northwest Trek. All three institutions have a history of supporting ongoing field conservation efforts through grant funding and donor contributions. Scientists with the Fort Lewis Fish and Wildlife Program are committed to conducting site monitoring on an ongoing basis as part of their core activities. 9. Is this proposal connected to a relevant AZA conservation/education program or institution? If so, explain how. LIMIT 750 CHARACTERS with spaces The Oregon spotted frog is considered endangered in Washington state and is a strong candidate for listing under the Federal Endangered Species Act. This project reflects the overriding goal of AZA s Amphibian Conservation Program; that every AZA-accredited zoo and aquarium, no matter how large or small, commit to saving at least one species of endangered amphibian. Furthermore, this project reflects the priorities of the Amphibian Taxon Advisory Group s Declining Amphibian Regional Response Plan, which proposes that AZA institutions commit to creating space for and channel resources to support North American frog species in particular. Continue to Questions next page

9 10. Are there collaborating institutions or organizations involved in this proposal (aside from the P.I. institutions)? Yes No A collaborating institution is defined as any institution actively involved in the design or completion of the project. Funding institutions not actively involved in project design or completion should be listed in the Other Support section of the Project Budget and need not be listed as collaborators. List all collaborating institutions below, including those associated with the personnel listed in Question 5, and attach one Statement of Institutional Support form for each collaborating institution to your CEF Application (see CEF Application Instructions). This form is available on the AZA website at Applications that do not include Statement of Institutional Support forms for all collaborating institutions listed will not be considered for funding. Individually crafted letters of support cannot be accepted in lieu of this form. Collaborating Institution Names: Washington Department of Fish and Wildlife Statement of Institutional Support Form Attached? (required) Fort Lewis Statement of Institutional Support Form Attached? (required) Statement of Institutional Support Form Attached? (required) Statement of Institutional Support Form Attached? (required) Statement of Institutional Support Form Attached? (required) 11. Have all the necessary permits been obtained for the project? Yes No Not Applicable a) If yes, list the permits here and attach one electronic copy of each to your CEF Application . Attachments should be titled Permit. N/A b) If the necessary permits have not yet been obtained, please explain why. N/A 12. If research animals are to be purchased for this project, indicate the destination of the animal(s) after the completion of the research or provide justifications for euthanasia. LIMIT 750 CHARACTERS with spaces N/A

10 PREVIOUS CEF SUPPORT 2008 AZA CONSERVATION ENDOWMENT FUND Project Budget If any of this proposal s Principal Investigators listed on the Cover Sheet received previous CEF support, please complete the required information below for each. 1. Name of P.I.: N/A Year of award: Amount of Award: Project Title: Were all required reports (annual/final) submitted? Yes No 2. Name of P.I.: N/A Year of award: Amount of Award: Project Title: Were all required reports (annual/final) submitted? Yes No ** If you need to add additional awards, please add their information to a separate Word document and include it as an attachment titled Past CEF Projects with your CEF Application . PROJECT BUDGET All funding requests from CEF should be for one year only. Funding decisions are announced in early September and CEF funds are to be used for work from the announcement date forward. Costs incurred prior to the announcement will not be covered by CEF funds. Total Project Budget: $165,271 Total Requested from CEF: $29,956 (round to nearest whole dollar) OTHER SUPPORT List any other support already received and/or other grant applications currently under review for components of the project. Item for which funding was requested Staff time, training, some headstarting equipment at OZ, NWT, WPT Staff time, some headstarting equipment at OZ, NWT Staff time, headstarting equipment at NWT Staff time, headstarting equipment at OZ Staff time, headstarting facility construction at WPZ Headstarting Facility Site Prep and Utilities at Amount Requested Amount Received Date Expected (if not yet received) Grant/Matching Source $13,582 $13,582 Approved NW Zoo and Aquarium $10,046 Pending, expected August, 2008 Pt. Defiance Zoo, Conservation Committee $6,300 $6,300 Approved NWT In-Kind $1,025 $1,025 Approved OZ In-Kind $34,000 Pending, expected September, 2008 Geyer Trust $20,424 $20,424 Approved WPZ In-Kind

11 WPZ, travel to and from headstarting site Note: Recipients of CEF grants are obligated to inform the CEF Scientific Advisory Committee Chair of any pending or overlapping grants received. IF such grants are received, the CEF Scientific Advisory Committee may recommend that the CEF award amount be reduced or refunded.

12 DETAILED BUDGET The detailed budget should be separated into CEF and non-cef costs. List under each category the budget items and method of calculation for each (e.g., 2 persons x 10 days x $25/day), as well as probable sources of support. The following should be listed as separate budget items: airfares lodging vehicle rental (automobiles, planes, boats) fuel per diem equipment over $500 supplies, services (lab analyses, editing) pharmaceuticals shipping/postage printing personnel costs CEF does not fund the following: Tuition, university fees, or fringe benefits associated with graduate students or other collaborators; administrative costs (including institutional overhead and submission costs for the publication of journal articles); travel unrelated to completion of the project (conference presentation expenses, travel to meetings, etc.); planning meetings that define, rather than implement, conservation/management/education goals. CEF does not fund salaries for project personnel other than graduate students, technicians, and start-up positions established solely for the purpose of completion of the project. CEF Budget Items: CEF Budget Item Student Technician Stipend, to update Recovery Plan 300 Gallon Plastic Tanks Lids for Tanks Method of Calculation (e.g., 2 persons x 10 days x $25/day) $15/hr x 8hrs/day x 5 days/week x 12 weeks Was funding for this Total Cost item also requested from another source? $7,200 Yes No $220 x 20 (minus $525 received from another souce) $3,875 Yes No $500 x 20 (minus $300 received from $9,700 Yes No another source) 1,500 Gallon Cistern $2,000 x 1 $2,000 Yes No Mesh top for headstarting $2/square foot, 2,500 square feet $5,000 Yes No facility Travel, Oregon Zoo 12 cars x 290 miles x 50.5c/mile (08 $424 Yes No rate) Travel, NW Trek 12 cars x 70 miles x 50.5c/mile (08 rate) $1,757 Yes No TOTAL REQUESTED FROM CEF $29,956 (round to

13 nearest whole dollar) ** If you need to add additional budget items, please add this information in a separate word document and include it as an attachment with your CEF Application titled Additional Budget Items. Non-CEF Budget Items: Budget Item WPZ COSTS WPZ Staff Time (Headstarting) 300 Gallon Plastic Tank, Method of Calculation (e.g., 2 persons x 10 days x $25/day) Total Cost Funding Source for Item 4 hr/day, 163 days, $21.50/hour $14,000 Private Foundation $175 x 3 $525 NW Zoo and Aquarium without shipping Aquarium Supplies $10 x 3 $30 NW Zoo and Aquarium Hatching Tank $4 x 3 $12 NW Zoo and Aquarium Lids $100 x 3 $300 NW Zoo and Aquarium Pumps $100 x 3 $300 NW Zoo and Aquarium Thermometers $25 x 3 $75 NW Zoo and Aquarium Water Test Kit $50 x 1 $50 NW Zoo and Aquarium 8-foot chain link fence to surround headstarting tubs Site prep including grading, construction of access, providing water supply and infiltrated drainage Electrical supply to site and distribution within the facility including predator control. $10/foot, 200 linear feet $20,000 Private Foundation $10,000 WPZ In-Kind $10,000 WPZ In-Kind Training at Mt.View $650 NW Zoo and Aquarium Travel 12 cars x 80 miles x 50.5c/mile (08 rate) $484 WPZ In-Kind SEE ATTACHED SEE ATTACHED SEE ATTACHED SEE ATTACHED BUDGET JUSTIFICATION Please justify each line item listed in the CEF Budget Items above and explain briefly why the items listed are necessary for completion of the project and reasonable in cost. LIMIT 2,500 CHARACTERS with spaces All costs covered by CEF funds relate to implementation of conservation goals.

14 Tanks, lids, cistern and mesh requested from CEF are all necessary components of creating a headstarting facility at WPZ. P.I. Dana Payne has visited the Mountain View Conservation & Breeding Center (the facility on which this project is modeled) and has a thorough understanding of the elements required. Amounts listed were using Web research and in consultation with the zoo's Exhibits and Facilites Manager. Based on his experience purchasing items for similar projects he was able to derive our best estimate of the costs associated with establishing a full-scale headstarting facility at the zoo. Additional funding to create the facility will be requested from a private foundation, none of the amounts listed as pending or approved under "Other Support" and "Non-CEF Budget Items" overlap with our request to CEF. Travel costs will be incurred in the course of transporting eggs and metamoprhs between headstarting facilities and the release site. Federal rates were used to estimate travel costs. The stipend for the "student technician" who will work with WDFW to create the updated Recovery Plan does not represent staff cost, tuition, university fee, fringe benefit, or any other "non-funded" category of expense.