LAGUARDIA COMMUNITY COLLEGE CITY UNIVERSITY OF NEW YORK NATURAL SCIENCES SCB 260 - GENERAL MICROBIOLOGY Course Coordinator: Olga Calderon, PhD Contact Info: ocalderon@lagcc.cuny.edu or 718-482-5749 Office M-220A CATALOGUE DESCRIPTION An introduction to microorganisms found in nature, industry and disease. Students are introduced to virology, bacteriology, immunology, epidemiology, pathology and other related areas of microbial physiology. The laboratory will deal with the isolation and identification of common pathogenic and nonpathogenic organisms utilizing techniques of staining, culturing, fermentation reactions and microscopic inspection. PREREQUISITES SCB202 or SCB204 or SCB209 REQUIRED TEXTS LABORATORY MANUAL PAPER COPY NO ELECTRONIC COPY PERMITTED Title: Microbiology Laboratory Theory & Application, 2nd Edition Authors: Michael J. Leboffe, Burton E. Pierce Publisher: Morton Publishing Company Copyright: 2008 ISBN-10: 0895829479 ISBN-13: 978-0895829474 TEXTBOOK Title: Microbiology: An Introduction, Books a la Carte Plus MasteringMicrobiology, 12/E Authors: Gerard J. Tortora, Berdell R. Funke, Christine L. Case Publisher: Benjamin Cummings Copyright: 2010,2013,2016 ISBN-10: 0321793110 ISBN-13: 9780321793119 ISBN-loose-leaf: 9780133983722 ATLAS (highly recommended, but optional) Title: Microbiology: A Photographic Atlas for the Laboratory: 1st Edition Authors: Steven K. Alexander Ph.D., Dennis Strete. Publisher: Benjamin Cummings. Copyright: 2000 ISBN-13: 9780805327328 Grading Criteria Lecture Laboratory 4 Exams 4 X 10 each = 40% *4 post-lab quizzes 4 X 3 = 12% 3 HWs 3 x 3.33ea = 10% 4 pre lab quizzes 3 X 1= 3% 1 Paper 1 x 10 = 10% 1 Morph. Unknown 1 X 5 = 5% Individual Unknown 20% *The lowest grade for one quiz for lab will be dropped COURSE GRADE A = 93 100 C = 73 76.9 A- = 90 92.9 C- = 70 72.9 B+ = 87 89.9 D+ = 67 69.9 B = 83 86.9 D = 63 66.9 B- = 80 82.9 D- = 60 62.9 C+ = 77 79.9 F = <59.9 1
ATTENDANCE Attendance is mandatory based on the College s policy. Both lecture and laboratory! Attendance will be taken at each session. INSTRUCTIONAL OBJECTIVES 1. To introduce the student to the field of microbiology; showing its medical and economic importance. 2. To develop student skills in the area of diagnostic bacteriology and parasitology. 3. To show the importance of sanitation in controlling infection. 4. To develop a student awareness of epidemiology. PERFORMANCE OBJECTIVES 1. To acquire the techniques and manual dexterity necessary for the safe handling of potentially harmful organisms for his own and others protection. 2. To consider no bacteria completely harmless. 3. To use the methods and precautions essential in the collecting and handling of pathogenic and non-pathogenic specimens for microbiological examination. 4. To use practical, efficient methods of disinfection and sterilization wherever necessary. 5. To use identification methods necessary to recognize pathogens. 6. To recognize the importance of good public health departments and good sanitation in maintaining a healthful environment. 7. To realize the dangers of the spread of disease due to high-speed transportation and the value of the work of the World Health Organization and others. 8. To understand that the problem of controlling infectious disease is greater than that of attacking the harmful microorganisms involving the body's resistance or susceptibility, the body's defense and the problem of immunization. 9. To apply the precautions learned in the laboratory to daily living e.g. hands kept away from eyes and mouth, care of food to prevent contamination, prevention of spread of infection in the home, hospital, and the community. 10. To appreciate the value of microorganisms as experimental tools and biological models. EXAMINATION MAKE-UP POLICIES LECTURE EXAMS There are four (4) major exams. If you are absent for any exam, a make-up will be permitted on your own time. Make-ups for exams will be administered only after documented absence (doctor s note, etc.). The makeup exams tend to be considerably more difficult than the scheduled exam. LABORATORY QUIZZES If you are absent or late, there will be no make-ups permitted. However, the lowest quiz grade will be dropped when calculating your quiz average for laboratory. LABORATORY SAFETY (Dress code/ Personal Protective Equipment) Students participating in microbiology labs must have their own lab coat for every lab. No exceptions. No open-toed shoes are allowed in the lab. Gloves are required for standard lab procedures if proper hand hygiene is performed; however students should wear gloves if their hands have fresh cuts or abrasions. Proper hand hygiene involves thorough hand washing prior to and immediately after finishing handling microorganisms. Safety glasses/ goggles are not required for standard lab procedures but they are permitted. It is recommended that students do not wear contact lenses to lab to prevent the risk of contamination; instead corrective lenses should be worn underneath a pair of splash-resistant safety goggles. Students not following the laboratory dress code and safety guidelines will not be permitted to attend the lab. Use of cell phones in class is prohibited. Cell phones must be turned off or on silent mode during class. 2
POLICY ON CHEATING Instructors of this course are required to implement College policy regarding cheating on examinations and quizzes. A complete statement is available through student counseling services. A synopsis is as follows: If an instructor suspects a student of cheating, or any of the violations listed below, the instructor will inform the student of his or her suspicion, and a student/teacher conference will be held. At that conference the suspected violation and the instructor's intended penalty for the violation will be discussed. 1. Give the student a copy of the STUDENT HANDOUT ON LAGUARDIA ACADEMIC INTEGRITY POLICY (this document) and answer any questions the student may have. 2. Inform the student of the reasons for his or her suspicions and the intended penalties. These penalties may include, but are not limited to, the following: An "F" on the paper, quiz, assignment or examination involved and an "F" for the course. 3. If the student admits guilt, and agrees on the penalty, he/she should indicate so on the formal complaint. The instructor will then send the complaint to the Office of Academic Standing and impose the penalty. 4. If the student does not admit guilt or agrees to the penalty, the student/teacher conference will end and a hearing on the issue must be held. The instructor will then submit the complaint to the Office of Academic Standing, which will send a copy to the Dean of Students who will then begin disciplinary proceedings. If the records of the Office of Academic Standing indicate that the student has committed a similar offense before the Office of Academic Standing will: Send a copy of the complaint to the Dean of Students, who will begin disciplinary proceedings INCOMPLETE POLICY The following is the policy on incomplete grades. There will be no exceptions to the rule. An incomplete grade is assigned ONLY if ALL of the following conditions have been met: The student is missing only one or two assignments. The student has complied with the attendance policy. The student presents the instructor with documentation explaining the reason he/she is unable to complete the assignment before the end of the semester. A student will not be given an incomplete if the student disappears at the end of the semester. Both student and instructor must complete an Incomplete Contract. ADDITIONAL INFORMATION Office hours - Will be announced by your instructor Quiz dates/assignment due dates - The days and times of the quizzes and exams will be announced well in advance. College Calendar - In order to allow for school holidays, it is often necessary to hold classes on days of the week other than those originally scheduled. Consult the College Catalog for changes. Official Withdrawal Date - Consult the College Catalog for Official Withdrawal Date. Student Rights and Responsibilities - Consult the Student Handbook and College Catalog. 3
LECTURE OUTLINE TOPIC TEXTBOOK History of microbiology Ch. 1; Ch. 2 (p. 33); Ch. 3 (p. 58) Survey of Microbial Life Appendix D & F Functional Anatomy of Prokaryotic & Eukaryotic cells Ch. 4 (pp. 76-98) (Review pp. 98-end) Microbial Metabolism Ch. 5; (Review selected sections of Ch. 2) - Appendix A glycolysis and the Krebs cycle Microbial Growth Ch. 6 (pp. 157 167); (174 177) Exam #1 Classification of Microorganisms Ch. 10 The Prokaryotes: Domains Bacteria and Archaea Ch. 11 The Eukaryotes: Fungi, Algae, Protozoa and Helminths Ch. 12 (selected readings: Appendix F) Viruses, Viroids, and Prions Ch. 13; (Appendix F) Exam #2 The Control of Microbial Growth Ch. 7 Antimicrobial Drugs Ch. 20 Principles of Disease & Epidemiology Ch. 14 Microbial Mechanisms of Pathogenicity Ch. 15 Innate Immunity: Nonspecific Defenses of the Host Ch. 16 Exam #3 Adaptive Immunity: Specific Defenses of the Host Ch. 17 Practical Applications of Immunology Ch. 18 Biotechnology, Recombinant DNA Ch. 9 Disorders Associated with the Immune System Ch. 19 Exam #4 (during final exam week) 4
LABORATORY OUTLINE LABORATORY TOPIC LAB BOOK 1 2 Lab Safety (PowerPoint presentation & Handout) Aseptic Technique Isolation of bacteria by dilution techniques Start microscopy Microscopy Recording culturing results from last lab Lab Safety pages (pg. 1-6) Exercises 1-3 (theory), 1-4, 1-5, 1-6 (theory). 3-1 Exercises 3-1,3-2, 3-3, 3 4 5 6 7 8 Staining Techniques: Preparation of smears and simple staining Negative staining Gram staining Staining Techniques (cont.) Acid Fast Staining Structural Staining Staining Techniques (cont.) Morphological Unknown Morphological unknown report must be submitted at the end of this session Microbes in the environment Special media for isolating bacteria Oxygen and the growth of bacteria Temperature and ph response Microbial metabolism/biochemical tests (Part I) Recording results from Special media, Oxygen req., Temp and ph Microbial metabolism/biochemical tests (cont.) (Part II) Recording results from Microbial metabolism/biochemical tests (Part I) Exercises 3-4, 3-5, 3-6 Exercises 2-2, 2-3, 2-4 Exercises 3-7, 3-8, 3-9, 3-10, 3-11 (theory only) Exercise 1-1 Exercises 2-6, 2-7, 2-8, 2-9, 4-3, 4-4, 4-5, 4-6 Exercises 5-1, 5-2, 5-3, 5-4, 5-5, 5-6, 5-7, 5-8 Exercises 2-6, 2-7, 2-8, 4-3, 4-4, 4-5, 4-6 Exercises 5-9, 5-10, 5-11, 5-12, 5-13, 5-14, 5-15, 5-16, 5-18; 9-4 & 9-5 (theory only) Exercises 5-1, 5-2, 5-3, 5-4, 5-5, 5-6, 5-7, 5-8 9 10-12 Physical & Chemical Methods of Control: Ultraviolet Radiation Damage & repair Disinfectants and Antiseptics Antimicrobial Drugs Recording results from Microbial metabolism/biochemical tests (Part II) Individual Unknown Project Students must submit their unknown report together with a Gram reaction slide no later than week 12. Exercises 2-12 & 2-13 (theory only), 5-20 7-2, 8-2 Exercises 5-9, 5-10, 5-11, 5-12, 5-13, 5-14, 5-15, 5-16, 5-18 Exercises 9-1, 9-2, 9-3 (as applicable) Table A-6 (p529), Bergey s manual, online resources, etc 5